ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

International Journal of Pharmaceutical, Chemical and Biological Sciences received 3566 citations as per google scholar report

International Journal of Pharmaceutical, Chemical and Biological Sciences peer review process verified at publons

INDIRECT ORGANOGENESIS OF THE MEDICINAL PLANT SPECIES, CRYPTOLEPISGRANDIFLORAWIGHT (APOCYNACEAE) BY TISSUE CULTURE TECHNIQUE

Abstract

Author(s): R.Prema1,S. Paulsamy2*, J. Thambiraj2and M. Saradha2

In the present study, protocol for callus induction and regeneration for the medicinal plant species, Cryptolepis grandiflorahas been developed by employing tissue culture technology. Young leaf explant inoculated on MS medium containing the growth regulators, BAP and NAA at 2.0 and 1.2 mgl-1 respectively showed higher callus induction (81%). The amount of callus responded for shoot formation greater was (78%) when subcultured on to the MS medium containing BAP (2.0 mg/L) and GA3(0.5 mg/L). The elongated shoots were rooted well on half strength MS medium supplemented with NAA at 1.5 mg/L and IBA at 0.2 mg/L. Regenerated plantlet were successfully acclimatized and hardened off in green house condition with better survival rate of 70%

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