ISSN: 2249–9504
CAS CODEN: IJPCDX

INDIRECT ORGANOGENESIS OF THE MEDICINAL PLANT SPECIES, CRYPTOLEPISGRANDIFLORAWIGHT (APOCYNACEAE) BY TISSUE CULTURE TECHNIQUE

Abstract

Author(s): R.Prema1,S. Paulsamy2*, J. Thambiraj2and M. Saradha2

In the present study, protocol for callus induction and regeneration for the medicinal plant species, Cryptolepis grandiflorahas been developed by employing tissue culture technology. Young leaf explant inoculated on MS medium containing the growth regulators, BAP and NAA at 2.0 and 1.2 mgl-1 respectively showed higher callus induction (81%). The amount of callus responded for shoot formation greater was (78%) when subcultured on to the MS medium containing BAP (2.0 mg/L) and GA3(0.5 mg/L). The elongated shoots were rooted well on half strength MS medium supplemented with NAA at 1.5 mg/L and IBA at 0.2 mg/L. Regenerated plantlet were successfully acclimatized and hardened off in green house condition with better survival rate of 70%

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