ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

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STREPTOMYCES CHARTREUSIS STRAIN ACTM-8 FROM THE SOIL OF KODAGU, KARNATAKA STATE (INDIA): ISOLATION, IDENTIFICATION AND ANTIMICROBIAL ACTIVITY

Abstract

Author(s): Nooshin KhandanDezfully1,2* and Nagaraja Hanumanthu1

The actinomycetes strain designated as ACTM-8 isolated from unexplored soil samples of Madikeri Taluk in Kodagu district, Karnataka State (India). The strain of ACTM-8 characterized based on the polyphasic taxonomic analysis, such as cultural, morphological and biochemical characteristics as well as 16S rRNA gene sequence analysis, as Streptomyces chartreusis (accession number KJ024097). The strain evaluated for antimicrobial activity against test microorganisms in two steps: primary and secondary screening. It showed a broad spectrum of antimicrobial activity against both test bacteria and fungi. The ethyl acetate extract of strain ACTM-8 was the most effective against Fusarium graminarium (36.33 ± 0.57mm) followed by F.poea (33.1 ±0.11mm), F.sporotrichioiodes (30.26 ±0.57mm), F.equeseti (24.3± 0.17mm), F.nivale (18.33 ± 0.11mm), Enterobacter aerogenes (12 .16± 0.28mm), Bacillus subtilis (10.66± 0.57mm) and Staphylococcus aureus (10.33 ± 0.57 mm) respectively. Therefore, the isolate ACTM-8 represents as the potent broad spectrum antimicrobial compound , may provide a valuable resource for the development of novel compound for pharmaceutical applications

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