Author(s): Tambekar DH and VR Dhundale
The Lonar Crater Lake, situated in the Buldhana District of the Maharashtra State of India, is highly saline, alkaline and harbors various unidentified, unique haloalkaliphilic bacterial species which can be produces biotechnological important secondary metabolites. The present study deals with isolation, production and partial characterization of antibacterial substance producing bacteria from the alkaline Lonar Lake. Total twenty nine bacilli were isolated by using different enrichment media, out of which BW1(1) selected for antibacterial study and subjected to phenotypic and biochemical characteristics and identified as Oceanobacillus iheyensis on the basis of 16S rDNA sequencing.The strain was found to be potential antibacterial against E. coliand moderate against K. pneumoniae, S. typhi, S. aureus and poor againstP. aeruginosa, P. vulgarisand E. aerogenes. The study reveals that the haloalkaliphilic O. iheyensisproduce broad spectrum of antimicrobial agentswhich can be exploited for biotechnological potential and improve as promising sources for new antibacterial compound.