Author(s): E. Venkata Naga Raju* and G. Divakar
Pseudomonas aeruginosastrain isolated from poultry waste was tested for its abilities to hydrolyze the feather. The effect of different production parameters such as pH, temperature, carbon source, nitrogen source (Organic and inorganic) incubation time inoculum sizes and surfactants on keratinase production by the isolated bacterial strain was studied. The enzyme production was assayed in submerged fermentation (SmF) condition. The maximum keratinase production was observed with maltose (120±2.6 U/ml), yeast extract (90±3.6 U/ml), ammonium sulphate (61±3.0 U/ml), pH 7.0 (114±4.1), temperature 40°C (110±1.9), Tween-80 (104±2.8U/ml), inoculum size level 5% (112±2.0U/ml) and incubation time 48 hours (101.01± 0.56U/ml) in the production medium.