ISSN: 2249–9504
CAS CODEN: IJPCDX

POTENTIAL OF FOURIER TRANSFORM INFRARED SPECTROSCOPY (FT-IR) TO DETECTION AND QUANTIFICATION OF OCHRATOXIN A: A COMPARISON BETWEEN REFLECTANCE AND TRANSMITTANCE TECHNIQUES

Abstract

Author(s): A.Bozza1*, SM. Tralamazza1, JI. Rodriguez2, MBS. Scholz3, DT. Reynaud4, PR. Dalzoto1andIC. Pimentel1

Ochratoxin A (OTA)is a mycotoxin with nephrotoxic, potentially carcinogenic and immunotoxic effects, naturally found in agricultural products including coffee beans. Fourier Transform Infrared Spectroscopy (FT-IR) is a practical procedure for the detection of organic compounds in matter, particularly useful because of its accuracy, rapid response, ease of operation and for being a non-destructive method. The aim of this work was to compare transmittance and reflectance infrared spectroscopy for detection and quantification of ochratoxin A from fungi of Aspergillusgenera isolated from green coffee beans. The High Performance Liquid Chromatography (CLAE) analysis showed that all strains to the Circumdati section are producer of OTA, unlike the representatives Nigrisection, which did not producer detectable levels of OTA.The FT-IR analysis showed that for detection and quantification of ochratoxin A, both models, incorporating transmittance and reflectance data, showed good results. Thus, the application of FT-IR to the detection and quantification of ochratoxin A in fungal isolates showed itself to be a viable and useful methodology.

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