ISSN: 2249–9504
CAS CODEN: IJPCDX

OPTIMIZATION OF MEDIAFORMULATION FOR BACTERIAL ISOLATEMSB-6

Abstract

Author(s): MDAG.Chandra Sekhar1, E. Radha Krishna2, VSSL.Prasad Talluri2and CH. Murali Mohan2

The bacterial isolate MSB-6 collected from Visakhapatnam coast of Bay of Bengal, Andhra Pradesh, India has shown considerable activity against Proteusvulgaris MTCC1771, Bacillussubtilis MTCC441 and Escherichiacoli MTCC443.So the culture conditions for the production of bioactive compounds by MSB-6 are optimized by using these pathogens as standard. Galactose and Peptone are the best carbon and nitrogen sources respectively for the optimum growth of the bacterial isolate. The metabolite production is initiated after 24 h of incubation and reached its maximum after 48 h and decreased gradually thereafter. The fermentation yield is high at 350C and pH 7.0.

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