Author(s): S. Kanolkar* and T. Walke
A new, simple, safe, accurate and reproducible spectrophotometric analytical method was developed for the estimation of Prulifloxacin, from its tablet dosage form. In the present investigation, 2.0 M Sodium benzoate solution also known as Neuberg’s hydrotropic salt was employed as hydrotropic solubilizing agent to solubilize poorly water-soluble drug Prulifloxacin for its spectrophotometric analysis. The primary objective of the present investigation was to employ this hydrotropic solution to extract the drug from its dosage form, precluding the use of costlier organic solvents. Aqueous solubilities of this selected model drug was to a great extent in 2.0 M sodium benzoate solutions. Various organic solvents like methanol, chloroform, ethanol, acetonitrile, hexane and toluene are widely used to conduct the spectrophotometric analysis, but higher cost and toxicity prevents their frequent use. The results of the analysis were validated statistically and by recovery studies. The λ max was observed at 296nm.The drug follows Beer’s law in concentration range of 15-60 mcg/ml with coefficient correlation value of 0.992. The mean percent recovery was 100.49%.