ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

International Journal of Pharmaceutical, Chemical and Biological Sciences received 3566 citations as per google scholar report

International Journal of Pharmaceutical, Chemical and Biological Sciences peer review process verified at publons

METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ATAZANAVIR AND RITONAVIR IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Abstract

Author(s): Anusha Tiyyagura*, Ashwini Gunda, Annapurna ReneeChitturi, Aravind sai, Abbaraju prasana Laxmi and Avinash Kodoori

A simple, precise, specific and accurate reverse phase HPLCmethod has been developed for the determination of Atazanavir and Ritonavir in pharmaceutical dosage forms.Thechromatographic separation was achieved on Symmetry C8 (4.6 x 100mm, 5?m, Make: ACE)column using a mixture of Buffer:Acetonitrile (45:55) as the mobile phase at a flow rate 0.9ml/min. The retention time of Atazanavir and Ritonavir was 2.9 min and 4.1min.The analyte was monitored using UV detector at 235 nm.Results of analysis were validated statistically and byrecovery studies. The method was validated according to theICH guidelines with respect to linearity, accuracy, precision and robustness. The proposedmethod can be successfully usedto determine the drug contents of marketed formulation.

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