ISSN: 2249–9504
CAS CODEN: IJPCDX

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF RESVERATROL AND GALLIC ACID BY RP-HPLC

Abstract

Author(s): Monika Gupta and Amandeep Kaur

In this study, a simple, fast, accurate, precise, reproducible, reverse phase high performance liquid chromatographic method have been developed and validated for the simultaneous estimation of Resveratrol and Gallic acid in pure drug form. The chromatography was carried out on a shimadzu LC-2010 ATVP prominence liquid chromatograph and using shimadzu SPD10AVP UV-Visible detector, an C-18, Nucleodur C18 having dimensions 5µ (250×4.6 mm) column used as stationary phase, with a mobile phase consisting of Phosphate buffer pH 3 ±0.02 pH adjusted with ortho phosphoric acid and methanol and acetonitrile (50: 30: 20 v/v) at a flow rate of 1 ml/min and detection wavelength of Resveratrol and Gallic acid at 306 nm and 263 nm, respectively. The retention time of resveratrol was found to be 4.75 minute and retention time of gallic acid was 2.15 minute respectively. The proposed method has been validated for accuracy, precision, linearity, robustness and range were within the acceptance limit according to International Conference on Harmonisation (ICH) guidelines. The method was found to be linear with r2 value of 0.998 for gallic acid and 0.999 for resveratrol. The calculated limit of detection (LOD) values were 0.689207 and 0.130961 µg/mL and limit of quantitation (LOQ) values were 2.088505 and 0.39685 µg/mL for resveratrol and gallic acid correspondingly. The developed method can be successfully employed for the routine analysis of Resveratrol and Gallic acid in API and Pharmaceutical dosage forms. Objective: The objective of present work was method development and validation of RP-HPLC for estimation of Resveratrol and Gallic acid.

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