Author(s): Prem Kumar Bichala, R. Suthakaran, Ali Lawal, Naveen Kumar, Ashok K Gurjar and Raman Singh
A new method was established for simultaneous estimation of Nevirapine and Lamivudine by RP-HPLC method. The chromatographic conditions were successfully developed for these parathion of Nevirapine and Lamivudine by using Develosil ODS HG-5 RP C18, 5?m, 15cmx4.6mm column, flowrate was1ml/min, mobile phase ratio was Potassium dihydrogen phosphate buffer (0.02 M, pH 2.5): Acetonitrile (57:43) (pH was adjusted with orthophosphoric acid), detection wavelength was 273 nm. The instrument used was Hitachi HPLC Auto Sampler, Separation module1575. The analytical method was validated according to ICH guidelines (ICH,Q2(R1)).The linearity study for Nevirapine and Lamivudine was found in concentration range of 1μg-5μg and 100μg-500μg and correlation coefficient(r2)was foundtobe 0.999and 0.999,%mean recovery was found to be100% and 100.5%,%RSD for repeatability was 0.2 and 0.4,%RSD for intermediate precision was 0.5 and 0.1 respectively.