Author(s): V. Jagannath Patro1, Nageshwara Rao. R2*and NK. Tripathy2
A rapid and sensitive liquid chromatography –tandem mass spectrometry (LC-MS/MS) method for determination of the Docetaxel in K3EDTA human plasma was developed and validated. Docetaxel was extracted from plasma by extraction procedure with using of MTBE as Liquid –Liquid extractive organic solvent. LC-MS/MS analysis using electro-spray ionization (ESI+) was performed on Thermo betaseal C-18 Column using as 10mM Ammonium acetate with 0.1% FA, Acetonitrile (60:40) as mobile phase. The method has a flow rate of 0.6ml/min. Retention of Docetaxel was 1.25 minutes. An excellent linearity (r2 .099) between the peak ratios and Docetaxel concentrations over the range of 0.2-100 ng/ml of plasma was studied. The lower limit of detection for Docetaxel on mass was 0.2 ng/mL.