ISSN: 2249–9504
CAS CODEN: IJPCDX

ISOLATION AND SCREENING OF L?ASPARAGINASE PRODUCING BACTERIA FROM VISAKHAPATNAM SOIL SAMPLES

Abstract

Author(s): VSSL.Prasad Talluri, M.Bhavanaand SV.Rajagopal*

L-asparaginase (E.C No 3.5.1.1) is a potential anti-carcinogenic enzyme. Six different soil samples of Vishakhapatnam regions were screened for potential producers of L-asparaginase using modified czapek dox’s agar containing L-asparagine and phenol red indicator with pH 6.8. The strain isolated from slaughter house soil sample (BSH-3) showed the maximum activity. The selected strain was characterized by biochemical tests as per the bergey’s manual of determinative bacteriology and was found to be Myroidesspecies. The enzyme production was carried out by submerged fermentation. The maximum enzyme activity was 85.7 IU/mg is showed by 24 hrs culture at 37oC.

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