Abstract
Author(s): Rahul Paruchuri, Shagun Trivedi*, Sandeep Vijayakumar Joshi, Gargeyi Pavuluri and Senthil Kumar. M
The goal of the present investigation was to formulate, optimize and characterize poly lactide -o - glcolic acid (PLGA) nanoparticles of Irinotecan for cancer therapy. Here the nanoparticles formed are based on solvent evaporation technique by using aqueous and organic phases. The formulation optimization is also carried out optimizing its various process and formulation parameters. The analytical method development is carried out using acetonitrile and phosphate buffer saline. Different organic solvents were tried and various surfactants were used to optimize the nanoparticulate formulation. The size range and zeta potential was measured using Malvern zeta seizer. The lyophilization was carried out using two different cryoprotectants. The maximum percent drug entrapment was found out to be 37.2%. The in vitro drug release of IRN NP was also found out using dialysis method in phosphate buffer saline pH 7.4. The in vitro drug release showed sustained release of drug over 24 hours. Hence the IRN loaded PLGA nanoparticles have potential as a drug delivery system. Furthermore, they may have utility for site-specific drug delivery since the small size of the particles may allow their delivery to extra vascular target sites through the leaky endothelia of inflamed and cancerous area