Author(s): Sayari Banerjee* and ChinmoyGhosh
It has been demonstrated by numerous studies that there is a slow decline of antioxidant status in older subjects with simultaneous increase in oxidative stress which is associated with increased production and accumulation of reactive oxygen species in elderly people.The present hospital based non-interventional cross-sectional study was designed to compare age-related oxidative stress as well as antioxidant status in middle aged (35-55y) and older (>60y) of Kolkata and its suburbs by measuring serum Thiobarbituric acid reactive substances ,serum protein carbonylation,vis-à-vis antioxidant defence with estimation of plasma superoxide dismutase. The Measurement of concentration of protein carbonylation and Thiobarbituric acid reactive substances in serum helps to identify the impact of age related oxidative stress.Superoxide dismutase is an antioxidant enzymes that catalyzes the dismutation of superoxide into oxygen and peroxide.Estimation of superoxide dismutase in plasma is important to evaluate the antioxidant status. Effect of age related oxidative stress and antioxidant parameters were investigated in 40 elderly people >60years attending geriatric depatment of the hospital for counseling (but otherwise healthy) and also in 40 middle aged persons(35-55y). On the basis of results obtained from this study it was evident that serum protein carbonylation and concentration of serum Thiobarbituric acid reactive substances were significantly higher in older subjects.It has been observed that enzymatic antioxidant serum Superoxide dismutase was significantlylower in elderly age group than middle aged group.The present study provide some important information regarding age related oxidative stress in healthy elderly population compared to middle aged subjects.