ISSN: 2249–9504
CAS CODEN: IJPCDX

ESTABLISHMENT OF LONG TERM PROLIFERATING SHOOT CULTURES OF COMMELINA BENGHALENSIS L. A MEDICINAL PLANT

Abstract

Author(s): Commelena benghalensis, medicinal plant, Plantlets and Regeneration.

Regeneration of Plantlets through nodal explants has been achieved in medicinal plant Commelina
benghalensis of commelinaceae, belongs to monocot. Green house grown explants were used to
control browning due to phenolic exudation. Plantlets obtained by nodal explants on Murashige and
Skoog’s (MS) medium fortified with 1.0 mg l-1 BAP (6, Benzyl amino purine) produced 06-10
multiple shoots. Cut ends of nodal stem segments rapidly turned brown and cultures failed to
establish. When 50 mg l-1 ascorbic acid and 25 mg l-1 Polyvinyl pyrrolidone (PVP) were added to
the medium, explants remained healthy, and cultures were established. Rooting of micro shoots
was achieved on half or full strength MS medium with 0.5-2.0 mg l-1 NAA (naphthalene acetic acid)
for 15 days. Regenerated plantlets acclimatized showed normal morphological characteristics
when transferred to soil. Survival of transplants was 95-99 % under green house condition.

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