ISSN: 2249–9504
CAS CODEN: IJPCDX

DIRECT AND DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF COPPER (II) IN MILK, BLOOD AND WATER SAMPLES

Abstract

Author(s): K.Sarasubudhi, S.Sivaramaiah and K.Ashok rao*

Copper is an essential micronutrient for human bodies. The accurate data in the distribution and the concentrations of Cu (II) in milk, blood and different organs and tissues is not thoroughly explained yet. Further issues which need clarification are the exact composition and constitution throughout the lactation is important. Breast milk contains the entire essential nutrients needed by neonates for growth development and maintaining of healthy condition. The physiological impacts of copper and copper enzymes are biomarkers in maintaining the sickness and health. Researches continue in an attempt to develop analytical method for the determination of copper in milk, blood and in drinking water, these discussions justify the purpose of our research related to studying the trace element Copper. In the paper a selective reagent {5M, 3H-B R} 5-{α Methyl -3 Hydroxy Benzylidene} rodanine was suggested for the development of simple and sensitive direct and derivative spectrophotometric method to the quantification of copper. In sodium acetate and acetic acid buffer at PH 5.5. The maximum absorbance was measured at 430nm. The Beer’s law is obeyed in the range of (0.05μg -13μg/ml). The molar absorptive (ε) and the Sandal’s sensitivity of the complex were 0.6027X104mole/cm and 0.01054μgcm-2respectively. The breast milk obtained from ten lacting mothers not using any hormonal contraceptive devises aged 19to26 years in Nellore town collected and analyzed similarly cow milk,buffeloe’s milk, blood samples, water samples were analyzed and an analytical concentration was made by comparing with WHO parameters.

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