ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

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DEVELOPMENTOFUV ?VISIBLE SPECTROPHOTOMETRIC METHOD FOR THE ANALYSIS OF METHOTREXATE IN TABLET FORMULATIONS

Abstract

Author(s): SU.Gadve*, PP. Hulle,MB.Shelke andMT. Mohite

Two simple, precise and economical UV methods have been developed for estimation of Methotrexatein bulk formulation. Method A involves measurement of UV absorbance in zeroorder derivative at 259nm. Method B deals with area under curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 256-262nm. The drug followsBeer-Lambert’s law in the concentration range of 3-10μg/ml inboth the methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed methods can be successfully applied for estimation of Methotrexatein routine analytical work

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