ISSN: 2249–9504
CAS CODEN: IJPCDX

DEVELOPMENT OF UV SPECTROPHOTOMETRIC FIRST ORDER DERIVATIVE METHOD FOR THE SIMULTANEOUS ESTIMATION OF ABACAVIR AND LAMIVUDINE IN COMBINED TABLET DOSAGE FORM

Abstract

Author(s): VP. Nagulwar, KP. Bhusari

Simple, accurate, precise, economical and reproducible analytical method has been developed for the simultaneous estimation of abacavir and lamivudine in pure bulk drug and in combined tablet dosage form by UV spectrophotometric first order derivative method. The stock solutions were prepared in mixture of acetonitrile and methanol followed by the further required dilutions with distilled water. In the first order derivative method, the wavelengths at which abacavir and lamivudine were analyzed were 229.2 nm and 284.8 nm respectively. At 229.2 nm abacavir has absorbance while lamivudine shows zero absorbance. Similarly, at 284.8 nm lamivudine shows absorbance while abacavir has zero absorbance. Thus both the drugs do not interfere in the quantitation of one another. Calibration graphs were obtained by the concentration ranges of 5-25 ?g/ ml of both the drugs. In bulk drugs, abacavir was estimated as 100.05%, lamivudine 100.11% whereas in the marketed tablets abacavir was found as 99.01% lamivudine 99.09% respectively. The results of analysis have been validated as per ICH guidelines and were found to be satisfactory. Hence, present study gives excellent methods for the determination of both the drugs in combined tablet formulation.

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