ISSN: 2249–9504
CAS CODEN: IJPCDX

DEVELOPMENT OF NEW COLORIMETRIC METHOD AND VALIDATIONFOR DETERMINATION OF LOPERAMIDEIN BULK AND MARKETED FORMULATION

Abstract

Author(s): Raval Kashyap* and Kevin Makavana*

In the present work, simple, sensitive, specific, and validated colorimetric method has been developed for the quantitative estimation of Loperamide in bulk and pharmaceutical dosage form. Loperamide in presence of acidic medium reacts with excess amount of Potassium bromide bromate and remaining Potassium bromide-bromate reacts with crystal violet to produce blue colour. The λmaxwas found to be 590nm for assay. The linearity was found in concentration range of 3-18 μg/ml. The correlation coefficient was found 0.999. The regression equation was found as Y= 0.030X + 0.006. The method was validated for linearity, accuracy, precision and ruggedness. The LOD and LOQ for estimation ofLoperamide were found as 0.09784, 0.2951respectively. Recovery of Loperamide was found to be 96.93%. Proposed method was successfully applied for the quantitative estimation of Loperamide in marketed formulations.

Get the App