ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

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DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF MICAFUNGIN IN BULK AND PHARMACEUTICAL FORMULATIONS

Abstract

Author(s): SM. Anil Kumar* , A. Satish Kumar Shetty and ND. Satyanarayan

The aim of present work was to develop an simple, accurate and precise zero order derivative method and First order derivative method for the estimation of Micafungin and validation according to ICH Q2(R1) guidelines. The absorbance maxima was determined at 269nm using Distilled Water .First order derivative spectrophotometric method has been developed at wavelength 249nm. The methods represented the correlation coefficient R2 = 0.999 for zero order method and R2 = 0.999 for First order derivative at concentration range 8-40µg/ml for both methods.The methods were validated for the parameters such as linearity, accuracy, precision, limit of quantification & limit of detection as per ICH guidelines. The results suggested that the proposed methods were found to be cost effective, sensitive, precise and accurate and hence can be successfully applied for the routine analysis of Micafungin in bulk and formulations

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