ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

International Journal of Pharmaceutical, Chemical and Biological Sciences received 3566 citations as per google scholar report

International Journal of Pharmaceutical, Chemical and Biological Sciences peer review process verified at publons

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AZITHROMYCIN AND LEVOFLOXACIN IN COMBINED TABLET DOSAGE FORM

Abstract

Author(s): P. Madhurima*, G. Tulja Rani

The aim of the present study is to develop a simple, accurate, precise, sensitive, less expensive and less time consuming method RP-HPLC for simultaneous determination of azithromycin and lovofloxacinin combined tablet dosage form. The method was validated for parameters like accuracy, linearity, precision, specificity, robustness and system suitability. The column efficiency has determined is not less than 3000 USP plate count and tailing factor is not more than 2.0. The %Relative standard deviation for the peak areas of the 6 replicate injections is not more than 2.0%. The % RSD of assay of 6 replicate injections was found to be within the limits. The recovery results indicating that the test method has an acceptable level of accuracy. The correlation coefficient met the acceptance criteria of NLT 0.999. The LOD and LOQ values from the study demonstrate that the method is sensitive. The system suitability parameters found to be within the limits for a change in temperature and flow rateand from the results it is concluded that the method is Robust. 

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