ISSN: 2249-9504
CAS CODEN: IJPCDX

Abstracted and Indexed

open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing open access publisher, open access publishing, open access journals and articles, peer reviewed open access journals, insight medical publishing
+32 25889658
Google Scholar citation report
Citations : 3566

International Journal of Pharmaceutical, Chemical and Biological Sciences received 3566 citations as per google scholar report

International Journal of Pharmaceutical, Chemical and Biological Sciences peer review process verified at publons

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF BENFOTIAMINE IN BULKAND DOSAGE FORM

Abstract

Author(s): B.Pavan Adithya*and M.Vijayalakshmi

A new simple, precise, sensitive and validated RP-HPLC method wasdeveloped for the estimation of Benfotiamine in bulk and pharmaceutical dosage form. The chromatographic conditions used for the separation was Phenomenex Luna C18 (4.6x250mm,5μ) and mobile phase comprised of acetonitrile : methanol : water: 0.1% OPA (40:20:35:5 v/v). The flow rate was 1.0 ml/min with detection at 249 nm. The retention time was found to be 3.84 min.The linearity was found to be in the range of 5-35 μg/ml for benfotiamine with correlation coefficient of 0.9999. The proposed method is accurate with 99.278% -100.791 % recovery and precise (%RSD of repeatability, intra-day and inter-day variations were 0.53, 0.45-0.67, 0.58-0.79).The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.1448 and 0.4388 μg/ml respectively.The method was successfully applied to pharmaceutical formulation because no chromatographic interferences from tablet excipients were found.

Get the App