Author(s): K. Sridevi Ranjitha and A. Lakshmana Rao
A simple, rapid and precise reverse phase high performance liquid chromatography method has been developed and validated for the determination of tirofiban in pharmaceutical formulation. Chromatography was performed by HPLC 2695 Waters Separation Module with Develosil C8 column 4.6×250 mm, with 5 µ particle size and the column maintained at ambient temperature. The injection volume was 20 µl and the total run time was 10 min. The detection was carried out at 277 nm. The mobile phase consisted of 1-octane sulfonic acid buffer (pH 3.0) and acetonitrile (55:45 v/v). Prepared mobile phase was filtered through 0.45 µ membrane filter and sonicated. Sample solution was prepared by dissolving the contents of the vial in diluents. The mobile phase was delivered at a flow rate of 1 ml/min.