ISSN: 2249–9504
CAS CODEN: IJPCDX

DEVELOPMENT AND VALIDATION OF A RP-HPLC METHOD FOR IDENTIFICATION AND ESTIMATION OF GALLIC ACID AND PROTOCATECHUIC ACID IN TRIGASORNMAS RECIPE

Abstract

Author(s): S. Settharaksa*, P. Pathompak, W. Saingam, F. Madaka, K. Chakree, L. Charoenchai

A simple, precise, accurate RP-HPLC method was developed for the quantitative estimation of gallic acid and protocatechuic acid in Trigasornmas recipe. The separation was accomplished with a column RP-C18 (250 mm x 4.6 mm x 5 micron) using a gradient of acetonitrile and 0.1% acetic acid in water as the mobile phase, with a flow rate was set to 0.9 mL /min and injection volume of 20 µL, analysis was screened with UV detector at 280 nm. The retention time for standard gallic acid was found at 9.28±0.05 minute and for the protocatechuic acid was found at 17.67±0.03 minute. Calibration curve was linear over the concentration range 1.56-100 µg/mL. Correlation coefficient (r 2 ) was 1.0000. The proposed method was validated for linearity, accuracy, selectivity, precision, LOD and LOQ. The proposed RP-HPLC method was found to be simple, precise, accurate and can be used for the quality control of the raw material as well as its formulation

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