Author(s): Ashish Kumar Sharma1 and Manas Mathur
The present investigation has been performed to study effects comparative analysis of both biologically reduced and chemically synthesized nanoparticles on toxicities assays which shows its effects on male reproductive system. Albino Wistar male rats (Rattusnorvegicus), healthy fertile 3–4 months old weighing about 150–180 g were used for experimentation. NPs synthesis by both chemical and green route method and injected intra-peritoneal in healthy fertile male rats at the dose level of (4mg and 8mg/kg ) for 60 days. The characterization data has been validated by UV, SEM, TEM and XRD (data not shown). Animals were killed 24 h after withdrawal of treatment, and sperm, haematological, serum, tissue biochemistry, and histopathological analyses were performed. NPs were synthesised and their size confirmed by images like SEM, and TEM. Sperm analysis altered, hormonal, biochemical, and histological changes support its antispermatogenic effect on reproductive organ. Decrease in sperm density and motility, fertility index, testes and accessory organ weight, tissue fructose, and glycogen contents in testes were observed. Increased cholesterol level and decrease testosterone level showed its adverse effect on fertility. Histological degradation in testes is clearly visible. No significant alteration in body and vital organs weight was found. NPs were synthesised successfully and revealed contraceptive activity in male rats.