ISSN: 2249–9504
CAS CODEN: IJPCDX

CELLULASE PRODUCTION OPTIMIZATION USING CELLULOLYTIC BACTERIA

Abstract

Author(s): P. Nisha*

Cellulase enzyme is an important enzyme used in various industries and is very expensive one. Cellulase enzyme is easy to produce from micro organisms. Optimum environmental conditions are varies for each organism for the enzyme production. This study is aimed that, maximum cellulase enzyme production from Micrococcus spbychanging some of the environmental parameters, enzyme activity assayed by Di Nitro Salicylic acid method with glucose as standard. Theparameters studieswere incubation temperature, pH, different concentrations of CMC and incubation time .It was found that, the optimum parameters for higher production of Cellulase enzyme was at 370C of incubation temperature, pH 8,1.5% of CMC and 72 hr of incubation time,0.9490IU/L. 

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