ISSN: 2249–9504
CAS CODEN: IJPCDX

BIOCHEMICAL CORRELATION BETWEEN NEONATAL HYPERBILIRUBINEMIA AND OXIDATIVE STRESS

Abstract

Author(s): Lekha Biswas1 and Arghya Ray Chaudhuri2*

Neonatal hyperbilirubinemia is known as the visible clinical manifestation of yellowish discoloration of skin and sclera, during the neonatal period, resulting from deposition of bilirubin in the neonatal bodies. Jaundice is observed during the 1st week in approximately 60% of term neonate and 80% of preterm neonate. In present study, we try to investigate and to determine the possible correlation between serum bilirubin levels with oxidative stress and antioxidant status in the Neonatal Hyperbilirubinemia patient with age and sex matched controls. Measured Thiobarbituric acid reacting substance (TBARS) in the serum of neonate (with hyperbilirubinemia) & normal controls as parameters of oxidative stress. To measure reduced glutathione, superoxide dismutase of the neonatal Hyperbilirubinemia patient and normal controls as parameters of antioxidant status. SGPT, ALP also measured between case , control, Serum Thiobarbituric acid reacting substances (TBARS) react with thiobarbituric acid in an acidic medium to form a pink colour compound, this product is extracted in organic solvents such as butanol and its absorbance are read at 532nm.Serum Bilirubin is measured by Diazo Method. Alkaline Phosphatase is measured By CAMP dependent Kinetic Technique. Serum SGPT is measured by Kinetic Method. Reduced Glutathione is measured by Semiautomated DTNB Technique. Serum Superoxide Dismutase (SOD) by modified Kakkar Methods. SGPT, ALP Marker of hepatic injury between case, control. This is Hospital based case control study. Neonatal hyperbilirubinemia is most common in neonate.These correlation studies show increased level of bilirubin increases serum TBARS level (significant at the 0.01 level), increased SOD (Correlation is significant at the 0.05 level, decreased reduced glutathione (Correlation is significant at the 0.05 level.) Hyperbilirubinemia of neonate increases the parameters of oxidative stress, We suggest regular monitoring of these patients for these parameters to avoid impeding harmful effects of oxidative stress.

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