Author(s): Y. Sharmila Juliet, K. Kalimuthu* , V. Chinndurai, V. Ranjitha and A. Vanitha
The present study was aimed to compare the anti-inflammatory and antidiabetic property of wild and micropropagated Cadaba fruticosa ethanol and methanol extracts. For albumin denaturation assay the highest inhibition percentage (60.82±0.54) was observed in WCFE followed by MCFE (57.28±0.92), WCFM (54.62±0.85) and MCFM (53.29±0.25) extracts at 1000 µg/ml concentration. The amount required for fifty percent inhibition (IC50) was found to be 880.04, 750.42, 670.31 and 610.21 for WCFE, MCFE, WCFM and MCFM. For HRBC membrane stabilization assay WCFE with 62.15. MCFE with 58.28, WCFM with 57.24 and MCFM with 54.28 percentage inhibitions was observed at 1000 µg/ml concentration. The IC50 values were found to be 324.14, 880.15, 840.08 and 710.11 for WCFE, MCFE, WCFM and MCFM extracts. The highest percentage of α-amylase assay 60.29±0.64, 55.87±0.32, 55.45±0.43 and 54.87±0.22 was observed at 500 µg/ml concentration of WCFE, MCFE, WCFM and MCFM respectively. The IC50 value of the extracts were 383.43, 432.8, 422.5 and 458.61 µg/ml respectively. The result of the present study indicates that all the four extracts showed the maximum α-glucosidase inhibiting activity at 500 µg/ml concentration with IC50 value 417.61, 464.45, 451.55 and 471.44. Cadaba fruitcosa extracts could remarkably inhibit the activity of αamylase and α-glucosidase. These results provide a rational for the use cadaba fruticosa to treat diabetics and inflammatory. The effect of wild and micropropagated plant on anti-inflammatory and anti-diabetic activity are almost similar, so instead of wild plant, the micropropagated plant can be used for further use.