Author(s): Erumalla Venkatanagaraju*, Rajshree Roy Chowdhury, Namitha Tony, Anthony Samuel, Yarram Sreelekha, Rashmita V and FR. Jobi Xavier
Extra cellular agarases are in great demand in lipid peroxidation, hydroxyl radical scavenging activity by generating agaro oligosaccharides and protoplast isolation. Most of the marine microbial flora have evolved to produce extra cellular agarases to utilize agar as a carbon source. In order to produce these enzymes in industrial scale, researchers were exploring non marine sources like soil, lake, hot spring to screen potent agarolytic enzyme producing microbial flora and cloning agarose promising gene into industry feasible microorganisms. Purification of extra cellular agarase has led to the determination of its 3D structure, which gives a better sight and understanding about its properties, and function. Purification is a multistage process involving the use of various separation techniques. Current review helps to give a wider view on the methods employed by different researches for the purification of extracellular agarase, produced by non-marine and marine environment microbiota.