ISSN: 2249–9504
CAS CODEN: IJPCDX

A STUDY ON CHANGE IN CONFORMATION OF TUBULIN ON INTERACTING WITH PROPOFOL VIA TIME OF FLIGHT MASS SPECTROSCOPY

Abstract

Author(s): D. Priscilla Jessy* and Pushpa Sahni

The present study revels to determine the change in secondary structure of protein when an anesthetic drug is added to it. The protein used was tubulin (20µg), which is the building block of MT. The change in conformation was observed in the presence and absence of 1ml of solution of tubulin glycerol buffer and propofol along with 0.1% TFA at 4°C via MALDI TOF MS (ABScix 4800). The result the mass spectrograph showed base peak at 53329.5 (m/z) in the absence of propofol which is approximately near the mass of tubulin. The other base peak, was observed at 72526.9 (m/z) indicating change in tubulin in presence of propofol. Based on the present study, we conclude that propofol hinders the polymerization of tubulin by changing its secondary structure and thus effect the consciousness in brain

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