Author(s): AM. Atta, S. Mostafa, I. Salama and MS. Gomaa*
In this study, we have developed, optimized and validated a new high-performance liquid chromatographic method for simultaneous determination of atenolol and pregabalin in their dosage forms and in human urine. The new method used a C18 column with mobile phase consisting of potassium dihydrogen phosphate and methanol in the ratio of 92.5:7.5 (v/v) and buffered at pH 6.0 with orthophosphoric acid, with a flow rate of 1 mL/min. Quantitation was achieved with UV detection at 230 nm. In addition, the urinary excretion patterns of atenolol and pregabalin were calculated using the proposed method by analysis of urine samples of five healthy volunteers during 24 hours after single oral administration of both drugs. The developed method was proved to be specific, robust and accurate for the determination of the studied drugs in pharmaceutical preparations and in human urine.