ISSN: 2249–9504
CAS CODEN: IJPCDX

STUDY OF ENHANCED LIPASE PRODUCTION USING AGRO-WASTE PRODUCT BY BACILLUS STEAROTHERMOPHILUSMTCC 37

Abstract

Author(s): Sasmita Sabat*, V. Krishna Murthy, Pavithra M, PalaMayurand Anitha Chandavar

The agro-waste products have been proved to be nutritionally enriched and thus can be used for production of many industrial products. This study reveals the utilization of renewable resources for their cost effective production and influence on the process under mild conditions.The physico-chemical conditions were optimised for the productivity. In the second experimental stage the best substrate in terms of carbon and nitrogen were estimated for the lipase production and activity. According to the optimum conditions the production and activity were measured as; at pH 7.518U/mL, temperature 550C, 17.181U/mL 18 hrs 9.495U/mL of incubation time. Maintaining these optimum conditions of physical parameters different carbon and nitrogen sources of 1.5% concentration were measured for activity. Wheat bran and cereal mixture produced maximum lipase activity of 22.04U/mL and 27.54U/mL respectively. To enhance the productivity 1% CaCl2 as an activator is added into the medium and it proved effective with an increase in the activity to 20.128U/mL.

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