Author(s): Padmavathi P. Prabhu*,ParamitaDas, Navaneet Krishna M and EVS.Subrahmanyam
The estimation of Guaiphenesin was based on the reaction of alcoholic solution of 1, 10-phenanthroline (Ferroin indicator) in the presence of ferric chloride. The orange red colour complex formed with ferric chloride and 1, 10-phenanthroline, due to oxidation of Guaiphenesin by ferric chloride and thereby itself undergoing reduction from ferric ion to ferrous ion. Ferrous ion forms a complex with 1, 10-phenanthroline which showed λmax at 510nm.The method obeys Beer-Lambert’s law in the concentration ranges of 10-60μg/ml of Guaiphenesin. The methods were validated for linearity, sensitivity, precision, accuracy, robustness and ruggedness.