ISSN: 2249–9504
CAS CODEN: IJPCDX

PURIFICATION, PROPERTIES AND KINETIC STUDIES OF CATALASE FROM LEAVES OF PHYLLANTHUS RETICULATUS

Abstract

Author(s): K.Beulah1* and T.Ramana2

Catalase (EC 1.11.1.6) an antioxidant enzyme has been screened in different medicinal plants, of which the leaf extracts of Phyllanthus reticulatus showed maximum activity of 784U/mg. Catalase from P.reticulatushas been purified 8 fold with 45% yield involving ammonium sulfate fractionation and gel filtration through SephadexG-150 column. The enzyme preparation is homogeneous on polyacrylamide gel electrophoresis and the molecular weight of the enzyme was estimated as 51.3kDa.The catalase exhibited optimum activity at pH 7.0 using 0.1M phosphate buffer and at temperature of 40°C.The enzyme was stable for 3 days at 4°C. The enzyme activity was slightlystimulated with CuSo4and MnCl2, where as it was slightly inhibited with NaCl and citric acid. The apparent Km value for the enzyme was determined as 0.74 Χ 105moles l-1

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