ISSN: 2249–9504
CAS CODEN: IJPCDX

PHYTOCHEMICAL INVESTIGATION AND CYTOTOXIC SCREENING OF LAGENARIA SICERARIASTANDLEY. FRUIT USING BRINE SHRIMPLETHALITY ASSAY MODELS

Abstract

Author(s): Suresh Ganapati Killedar, Sarang Sunil Mahamuni*, Harinath Nivruti. More, Sachin Shrikant Gadakari,Ashwini Balkrishna Nale and Anuja Annaso Pawar

Cancer is one of the leading causes of mortality worldwide. Many of the cucurbitaceae plants possess antitumor activity on the traditional use. The present study was carried out to evaluate the anticancer of activity of extracts Lagenaria sicerariaStandley Fruit. This fruit has the antioxidant activity so the plant may have anticancer activity. The present research had carried out on laboratory level assay to avoid the use of different animal models. Preliminary phytochemical tests of successive extraction of Lagenaria sicerariaStandely Fruit powder had performed to find out the different chemical moieties. Preliminary anticancer screening by exposure of different extractsBrine shrimp modelwascarried out to find out the lead extract which shows the promising cell growth inhibitory activity. The cytotoxic assay was selected because this is easy to done and give fastest promising results. Brine shrimp modelwere selected for the cytotoxic assay which shows the cytotoxicity that compared with standard antimitotic drug (Colchicine100PPM and100 PPM). n-Butanol extract of Lagenaria sicerariaStandley Fruit powder shows the promising anticancer activity orcytotoxicitythat’ so it is selected as a lead extract. Further isolation of active moiety from n-Butanol extract for anticancer activity by chromatographic techniques is almost completed.

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