Author(s): P.Rathakrishnan*and P.Nagarajan
The production of protease was optimized using groundnut shell as a substrate by Bacillus cereus. The nutrient variables were selected in accordance with the Plackett-Burman design. Four significant variables (NaNO3, Tryptone, K2HPO4and Malt extract) were selected for the optimization studies. The selected components were optimized using Response Surface Methodological approach. The optimum conditions are: NaNO30.0386g/gds,Tryptone0.0338g/gds,K2HPO40.2373g/gds and Malt extract 0.2090g/gds. A second-order model equation was suggested and then validated experimentally. The maximum protease production was 74.62U/gds.