ISSN: 2249–9504
CAS CODEN: IJPCDX

OPTIMIZATION OF MINERALNUTRIENTSUPPLEMENTS FOR THE PRODUCTION OF XYLANASEBY ASPERGILLUS NIGER UNDER SSF USING CENTRAL COMPOSITE DESIGN

Abstract

Author(s): K.Vimalashanmugam*and T.Viruthagiri

Xylanase production by Aspergillus Niger in solid state fermentation was investigated. Sugarcane bagasse samples collected from the local agricultural field was used as the source of carbon for the miroorganism. The effect of twelve nutrient supplements in the SSF medium were investigated using Plackett-Burman design and the nutrients namely Yeastextract, CuSO4 and MgSO4.7H2Owere found to be significant on the production of xylanase. The respective concentrations of the selected three nutrients were also optimized using central composite design of response surface methodology to obtain the maximum enzyme yield. The optimal concentrations were found to be yeast extract3.47450 mg/gds; CuSO4= 0.58665 mg/gds; MgSO4.7H2O = 0.94089 mg/gds. Under the optimum conditions the maximum xylanase yield of 399 IU/gdswas obtained experimentally.

Get the App