ISSN: 2249-9504
CAS CODEN: IJPCDX

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Citations : 3566

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NOVEL VALIDATED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF LISINOPRIL AND AMLODIPINE IN BULK AND TABLET DOSAGE FORM

Abstract

Author(s): V. Bhaskara Raju and A. Lakshmana Rao

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of lisinopril and amlodipine in tablets. A column having 150 × 4.6 mm i.d. in isocratic mode with mobile phase containing acetonitrile: phosphate buffer (60:40; adjusted to pH 3.0) was used. The flow rate was 0.5 ml/min and effluent was monitored at 215 nm. The retention time (min) and linearity range (μg/ml) for lisinopril and amlodipine were (4.111, 3.097) and (20-60, 10-30), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of lisinopril and amlodipine in tablets.

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