ISSN: 2249–9504
CAS CODEN: IJPCDX

NANOPARTICULATE DRUG DELIVERY SYSTEM OF POORLY WATER SOLUBLE ANTICANCER DRUG AND THEIR IN VITROCYTOTOXICITY STUDIES

Abstract

Author(s): M.KrishnaPillai1*and N.Thirumoorthy2

The objective of this investigation was to develop a microsphere and also attempts were made to control the release of silymarin. Multiple-unit of silymarin microspheres was prepared from Ethyl cellulose and EudragitRS100 polymer by using emulsion solvent evaporation method and Ionic cross linking technique. These microspheres were evaluated for Drug entrapment, Drug loading, Percentage yield Swelling index, scanning electron microscopy and in vitrodrug release.All formulationshowedtotal drug release duration more than 12 hours. It was observed that the result of in-vitrodissolution study revealthat the formulation ED3 & ES3 gave controlled release pattern. Silymarin microspheres (1:3ratio) showed cytotoxicity against HT-29 cells and DU145 Prostate carcinoma cellines. Hence the formulations can be effectively tested for its anticancer activity. The silymarin microspheres showed maximum release at 8 to 12 hours in pH 1.2 Maximum percentage cell viability is reduced up to 25 percentages at 12 hour meaning that 75 percentage cell deaths occurred in HT-29 cells and DU145 Prostate carcinoma cells. Most of the drug release wasobserved at 12hour andgood cytotoxicity.

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