Abstract
Author(s): MuruganT1*and Sridharan M2
The present study was carried out to obtain enhanced yield of Spirulina biomass and phycocyanin by media optimization with two species of Spirulina i.e... S.platensis(filamentous type) and S.platensis var lonar. Modified Zarrouk’s medium was used as the basal medium (it also served as control), in which different concentration of multivitamins was supplemented i.e., 250mg, 300mg, 350mg, 400mg, 450mg and 500mg/l was added. The cultivation was carried out in a 1000ml Erlenmeyer flask’s for a period of 15 days at room temperature with artificial aeration and lighting. The growth was monitored by Direct Microscopic Count (DMC), Optical Density (OD), and Dry biomass on daily basis till 15thday. On comparison of two species the S.platensisvar lonarshowed the highest biomass than the S.platensisin this study. The highest yield of S.platenisiswas 2.56g/l in 500mg/l supplements and the S.platensisvar lonarshowed 3.52g/l in 250mg/l multivitamin supplements. The highest C-phycocyanin content was observed with Spirulina platensisin 400mg/l supplementation (1004mg/ml) and in the control it was 480mg/ml. in terms of purity both species gave increased purity in the 300mg/l and 400mg/l supplements when compared to the control