ISSN: 2249–9504
CAS CODEN: IJPCDX

GC-MS ANALYSIS OF PHYTOCOMPONENTS AND TOTAL ANTIOXIDANT ACTIVITY OF HEXANE EXTRACT OF SINAPIS ALBA

Abstract

Author(s): Sujatha1, Karthika1, Sivakamasundari1, Mariajancyrani2* and Chandramohan2

To characterize the phytochemical constituents from Sinapis alba by using GC-MS method. Ten grams of the powdered sample mixed with 40ml of n-Hexane. This fraction was taken for GC-MS analysis. The analysis was carried out on a GC clarus 500GC system with a column packed with Elite-1(10% dimethyl poly siloxane, 30x0.25mm ID x 1EMdf) the compounds are separated using with Helium as carrier gas at a constant flow (1ml/min).Sample extract (2HL) injected into the instrument was detected by Turbo gold mass detector (Perkin Elmer) with the aid of Turbo mass 5.1 software. The GC-MS analysis provided peaks of different phytochemical compounds namely 1, 3, 5- Cyclo-heptatriene, octane, Hexanal, Heptanal, Furan, 2-pentyl octanal, Nonanal, Decanal, 4- Undecene,2-Tridecenal,2,4-Decadienal,2- Undecenal,2-Pentene,1-ethoxy-4,4-dimethyl,7-Tetradecene,Diethylphthalate,3- octadecenePentadecene,3-Hexadecene,3,7,11,15-Tetramethyl-2-hexadecen-1-ol,n-Butyl myristate,Sulfurous acid,2-prophl tride cylester,1-Hexadecanol,9,12-octadecadicn-1-ol,1- Hexadecanol,9,12-octadecadicn-1-ol,Hexadecanoic acid,butylester,Ethanone,2-cyclohexyl-1- (1H-imidazol-2-yl).Total Antioxidant activity was evaluated by Phosphomolybdenum assay method.

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