ISSN: 2249–9504
CAS CODEN: IJPCDX

DIFFERENT TYPES OF PCR TECHNIQUES AND ITS APPLICATIONS

Abstract

Author(s): S. Rajalakshmi

Polymerase chain reaction is a biological technology to produce ample number of DNA copies of a particular sequence. Three primary steps involved are de-naturation, annealing and extension. PCR techniques has a lot of applications in plant biology, diagnosis of influenza- human brucellosisSalmonellae, cloning purpose, in the field of Dentistry, microbiology, forensic science etc., There are many types of PCR techniques such as RT-PCR, touchdown PCR, real time PCR, nested PCR, multiplex PCR, semi quantitative PCR, assembly PCR, asymmetric PCR, LATE- PCR, dial out-PCR etc., This paper is an attempt to give a brief idea about the various types of PCR techniques

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