ISSN: 2249–9504
CAS CODEN: IJPCDX

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF GLYCYRRHETINIC ACID IN HYDRO-ALCOHOLIC EXTRACT OF GLYCYRRHIZA GLABRA

Abstract

Author(s): SK. Patil*, VR. Salunkhe and SK. Mohite

A simple, rapid, accurate, precise, and economic spectrophotometric method for estimation of Glycyrrhetinic acidin hydro-alcoholic extract of Glycyrrhiza glabra L.have been developed. Glycyrrhetinic acidshow absorbance maximum at 254 nm when Phosphate Buffer (pH-6.8) : Ethanol are used as solvent in 70 : 30 proportion, so absorbance was measured at the same wave lengths for the estimati1on of Glycyrrhetinic acid. Glycyrrhetinic acidobeys the Beer Lambert's law in the concentration range of 5-35μg/mL. A method was validated according to ICH guidelines and can be adopted for the routine analysis of Glycyrrhetinic acidin hydro-alcoholic extract of Glycyrrhiza glabra. The method is simple, rapid, safe, accurate, economical and useful for standardization of the licorice products

Get the App