Author(s): Nagakanyaka Devi Paladugu1*, G.Devala Rao2, Bonthu Satyanarayana3andDeepthi Poloju3
A simple, rapid, sensitive, reverse phase isocratic RP-HPLC method was developed for determination of Zolmitriptan. The method was carried out using SYMMETRY C18 packed column [3.5μm, 2.1x50mm] with mobile phase comprised of Acetonitrile: water (70:30 v/v) with 0.2 ml / min flow rate was quite robust. The optimum wavelength for detection was 225 nm at which better detector response for the drug was obtained. The run time was set at 5 min and the retention time was 1.04 minutes. The method was validated for specificity, accuracy, precision, linearity, and limit of detection, limit of quantification, robustness, solubility and stability. LOD and LOQ were found to be 0.5 ng/ml, 1ng/ml respectively. The calibration curve was linear in the concentration range of 0.03-1μg/ml with coefficient of correlation 0.994.