ISSN: 2249–9504
CAS CODEN: IJPCDX

DETERMINATION OF FOUR NEONICOTINOID INSECTICIDE RESIDUES IN COTTON SEED OIL USING MATRIX SOLID-PHASE DISPERSION COUPLED TO HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

Abstract

Author(s): Nageswara Rao.T

A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of neonicotinoid insecticide residues (Imadacloprid, Dinotefuran, Thiamethoxam and Acetamiprid) in cotton seed oil. The evaluated parameters included the type and amount of sorbent (silica gel, C18 and Florisil) and the nature of eluent (tetrahydrofuran, Millipore water and acetonitrile).The best results were obtained using 1.0 g of cotton seed oil sample, 1.0 g of C18 as sorbent and 20ml of tetrahydrofuran -Millipore water (2:8, (v/v)).The method was validated using in cotton seed oil samples spiked with insecticides at different concentration levels (0.03 and 0.3 μg/mL). Average recoveries (using each concentration six replicates) ranged 89-96%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-2.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 μg/mL and 0.03 μg/mL respectively.

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