Abstract
Author(s): VS. Pramitha* and N. Sree Kumari
Marine brown alga, Sargassumwightiiextracts was analyzedfor the anti-inflammatory and antioxidant activity by in vitromethods and the active ethyl acetate fraction has been subjected to GCMS analysis. At 50 μg/ml RBCs membrane showed an inhibition of 55.7% followed by methanol (54.3%) and hexane (52.2%), whereas in hypotonicity induced haemolysis, it is found toprotect the erythrocyte membrane against lysis induced by hypotonic solution compared to diclofenac sodium, a standard drug. It also showed maximum inhibition of 52.9% compared to methanol and hexane in its proteinase and albumin denaturationtest (70.8%) at 50 μg/ml. Free radical scavenging assay showed that ethyl acetate extract has significant scavenging effect on DPPH (64.9%) at 200 μg/ml. GC-MS analysis revealed that ethyl acetate fraction of S. wightii contains seventeen compounds and some of the major compounds detected wereBromoacetic acid, hexadecyl ester (94.98%),1,4-Eicosadiene (87.16%), Eicosane (73.97%), 6-Octadecenoic acid, (Z)- (72.17%), n-Hexadecanoic acid (62.97%), Benzene, 1,2-dimethoxy-4-(1-propenyl)- (62.92%), Stigmasta-5,24(28)-dien-3-ol, (3.beta)- (61.06%), Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3- (Phenyl methyl)- (56.62%) 2(1H)- Pyrimidinone, 4-amino-5-methyl-(41.97%) and 4-Methoxy-3- Propoxy-Benzaldehyde (40.18%).